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Institut für Physikalische Chemie

Lessingstraße 10
07743 Jena
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Gaurav Sharma


Tip enhanced Raman spectroscopy (TERS) is an analytical technique providing high specificity for material detection. It combines the high spatial resolution imaging power of an Atomic Force Microscope and specificity of Raman spectroscopy. Raman spectroscopy already finds application as an advanced analytical tool for biological and geological specimen. In this project we plan to investigate nanoscale surface alteration of mineralogical surfaces (black slate rock) by the fungus Schizophyllum commune. TERS setup is limited to transparent samples. Consequently the opacity of most geological samples is a major limitation. To overcome this obstacle we propose a non-optical feedback based experimental setup for the opaque geological samples with illumination & detection directly from above the TERS probe. Such a tuning fork based approach can potentially even improve the sensitivity and resolution compared to conventional AFM. A major challenge will be the adaption of appropriate plasmonic tips, as these probes are not commercially available. Major adaptions to the optical setup (optimization of NA, working distance and polarisation) will be required. In parallel an alternative, less sensitive, approach will be tested that will enable the investigation of model systems (cultured fungi on different substrates) in an early stage and allow the optimization of culture conditions with respect to the investigations





Home PhD projects Gaurav Sharma: Setup of a reflection mode TERS system for nanoscale spectroscopic investigation of microbial mineralization.